International Journal of Radiation Oncology * Biology * Physics
Volume 49, Issue 2 , Pages 373-377 , 1 February 2001

The use of DNA double-strand break quantification in radiotherapy

Presented at ICTR 2000, Lugano, Switzerland, March 5–8, 2000.

  • Trevor J McMillan, Ph.D.

      Affiliations

    • Department of Biological Sciences, Institute of Environmental and Natural Sciences, Lancaster University, Lancaster, UK
    • Corresponding Author InformationReprint requests to: T. J. McMillan, Department of Biological Sciences, Institute of Environmental and Natural Sciences, Lancaster University, Lancaster LA1 4YQ, UK. Tel: 44 (0) 1534 59 4486; Fax: 44 (0) 1524 843 854
    • ,
  • Simon Tobi, Ph.D.

      Affiliations

    • Department of Biological Sciences, Institute of Environmental and Natural Sciences, Lancaster University, Lancaster, UK
    • ,
  • Santiago Mateos, Ph.D.

      Affiliations

    • Departmento de Biologia Celular, Facultad de Biologia, Universidad de Sevilla, Seville, Spain
    • ,
  • Catherine Lemon (M.R.C.P., F.R.C.R.)

      Affiliations

    • Department of Oncology, Mount Vernon Hospital, Northwood, Middlesex, UK

,Accepted 31 August 2000.

References 

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  2. Lobrich M, Rydberg B, Cooper PK. Repair of x-ray-induced DNA double strand breaks in specific Not I restriction fragments in human fibroblasts (Joining of correct and incorrect ends). Proc Natl Acad Sci USA. 1995;92:12050–12054
  3. Olive PL, Banath JP. Detection of DNA double-strand breaks through the cell cycle after exposure to X-rays, bleomycin, etoposide and 125IdUrd. Int J Radiat Biol. 1993;64:349–358
  4. Olive PL, Durand RE, Jackson SM, et al.  The comet assay in clinical practice. Acta Oncol. 1999;38:839–844
  5. Woudstra EC, Roesink JM, Rosemann M, et al.  Chromatin structure and cellular radiosensitivity (A comparison of two human tumour cell lines). Int J Radiat Biol. 1996;70:693–703
  6. Olive PL. The role of DNA single- and double-strand breaks in cell killing by ionizing radiation. Radiat Res. 1998;150(Suppl.):S42–S51
  7. Mateos S, Peacock JH, Steel GG, McMillan TJ. Study of DNA double-strand break induction in different chromatin substrates of radioresistant and radiosensitive human tumour cell lines. Biomedical Letters. 1998;58:7–18
  8. Mateos S, Steel GG, McMillan TJ. Differences between a human bladder carcinoma cell line and its radiosensitive clone in the formation of radiation-induced DNA double-strand breaks in different chromatin substrates. Mutat Res. 1998;409:73–80
  9. Jeggo PA, Taccioli GE, Jackson SP. Menage a trois (Double strand break repair, V(D)J recombination and DNA- PK). Bioessays. 1995;17:949–957
  10. Thacker J. A surfeit of RAD51-like genes?. Trends Genet. 1999;15:166–168
  11. Whitaker SJ, Ung YC, McMillan TJ. DNA double-strand break induction and rejoining as determinants of human tumour cell radiosensitivity. A pulsed-field gel electrophoresis study. Int J Radiat Biol. 1995;67:7–18
  12. Nunez MI, McMillan TJ, Valenzuela MT, Ruiz de Almodovar JM, Pedraza V. Relationship between DNA damage, rejoining and cell killing by radiation in mammalian cells. Radiother Oncol. 1996;39:155–165
  13. Whitaker SJ, McMillan TJ. Pulsed-field gel electrophoresis in the measurement of DNA double-strand break repair in xrs-6 and CHO cell lines (DNA degradation under some conditions interferes with the assessment of DSB rejoining). Radiat Res. 1992;130:389–392
  14. Foray N, Monroco C, Marples B, et al.  Repair of radiation-induced DNA double-strand breaks in human fibroblasts is consistent with a continuous spectrum of repair probability. Int J Radiat Biol. 1998;74:551–560
  15. Eastham AM, Marples B, Kiltie AE, Orton CJ, West CM. Fibroblast radiosensitivity measured using the comet DNA-damage assay correlayes with clonogenic survival parameters. Br J Cancer. 1999;79:1366–1371
  16. Dikomey E, Dahm-Daphi J, Brammer I, Martensen R, Kaina B. Correlation between cellular radiosensitivity and non-repaired double-strand breaks studied in nine mammalian cell lines. Int J Radiat Biol. 1998;73:269–278
  17. McMillan TJ, Peacock JH. Molecular determinants of radiosensitivity in mammalian cells. Int J Radiat Biol. 1994;65:49–55

PII: S0360-3016(00)01467-X

International Journal of Radiation Oncology * Biology * Physics
Volume 49, Issue 2 , Pages 373-377 , 1 February 2001

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