International Journal of Radiation Oncology * Biology * Physics
Volume 49, Issue 2 , Pages 367-371 , 1 February 2001

Genetic manipulation of radiosensitivity

Presented at ICTR 2000, Lugano, Switzerland, March 5–8, 2000.

  • Adrian C Begg, Ph.D.

      Affiliations

    • Corresponding Author InformationReprint requests to: Dr. A. C. Begg, Division of Experimental Therapy, The Netherlands Cancer Institute/Antoni van Leeuwenhoek Hospital, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    • ,
  • Conchita Vens, Ph.D.

,Accepted 31 August 2000.

References 

  1. Kyprianou N, King ED, Bradbury D, et al.  bcl-2 over-expression delays radiation-induced apoptosis without affecting the clonogenic survival of human prostate cancer cells. Int J Cancer. 1997;70:341–348
  2. Brown JM, Wouters BG. Apoptosis, p53, and tumor cell sensitivity to anticancer agents. Cancer Res. 1999;59:1391–1399
  3. de Murcia G, Menissier DM. Poly(ADP-ribose) polymerase (A molecular nick-sensor). [published erratum appears in Trends Biochem Sci 1994 Jun;19(6):250] Trends Biochem Sci. 1994;19:172–176
  4. Jeggo PA. DNA repair (PARP—Another guardian angel?). Curr Biol. 1998;8:R49–R51
  5. Rudat V, Kupper JH, Weber KJ. Trans-dominant inhibition of poly(ADP-ribosyl)ation leads to decreased recovery from ionizing radiation-induced cell killing. Int J Radiat Biol. 1998;73:325–330
  6. Bristow RG, Jang A, Peacock J, et al.  Mutant p53 increases radioresistance in rat embryo fibroblasts simultaneously transfected with HPV16-Eqn. 7 and/or activated H-ras. Oncogene. 1994;9:1527–1536
  7. Bristow RG, Hu Q, Jang A, et al.  Radioresistant MTp53-expressing rat embryo cell transformants exhibit increased DNA-dsb rejoining during exposure to ionizing radiation. Oncogene. 1998;16:1789–1802
  8. Dent P, Reardon DB, Park JS, et al.  Radiation-induced release of transforming growth factor alpha activates the epidermal growth factor receptor and mitogen-activated protein kinase pathway in carcinoma cells, leading to increased proliferation and protection from radiation-induced cell death. Mol Biol Cell. 1999;10:2493–2506
  9. Reardon DB, Contessa JN, Mikkelsen RB, et al.  Dominant negative EGFR-CD533 and inhibition of MAPK modify JNK1 activation and enhance radiation toxicity of human mammary carcinoma cells. Oncogene. 1999;18:4756–4766
  10. Abbott DW, Holt JT. Mitogen-activated protein kinase kinase 2 activation is essential for progression through the G2/M checkpoint arrest in cells exposed to ionizing radiation. J Biol Chem. 1999;274:2732–2742
  11. Cartee L, Vrana JA, Wang Z, et al.  Inhibition of the mitogen activated protein kinase pathway potentiates radiation-induced cell killing via cell cycle arrest at the G2/M transition and independently of increased signaling by the JNK/c-Jun pathway. Int J Oncol. 2000;16:413–422
  12. Wright JH, Munar E, Jameson DR, et al.  Mitogen-activated protein kinase kinase activity is required for the G(2)/M transition of the cell cycle in mammalian fibroblasts. Proc Natl Acad Sci U S A. 1999;96:11335–11340
  13. Abrieu A, Fisher D, Simon MN, et al.  MAPK inactivation is required for the G2 to M-phase transition of the first mitotic cell cycle. EMBO J. 1997;16:6407–6413
  14. Smeets MF, Mooren EH, Abdel-Wahab AH, et al.  Differential repair of radiation-induced dna damage in cells of human squamous cell carcinoma and the effect of caffeine and cysteamine on induction and repair of dna double-strand breaks. Radiat Res. 1994;140:153–160
  15. Balaban N, Moni J, Shannon M, et al.  The effect of ionizing radiation on signal transduction (Antibodies to EGF receptor sensitize A431 cells to radiation). Biochim Biophys Acta. 1996;1314:147–156
  16. Huang SM, Harari PM. Modulation of radiation response after epidermal growth factor receptor blockade in squamous cell carcinomas (Inhibition of damage repair, cell cycle kinetics, and tumor angiogenesis). Clin Cancer Res. 2000;6:2166–2174
  17. Yang YM, Dolan LR, Ronai Z. Expression of dominant negative CREB reduces resistance to radiation of human melanoma cells. Oncogene. 1996;12:2223–2233
  18. Scolnick DM, Chehab NH, Stavridi ES, et al.  CREB-binding protein and p300/CBP-associated factor are transcriptional coactivators of the p53 tumor suppressor protein. Cancer Res. 1997;57:3693–3696
  19. Avantaggiati ML, Ogryzko V, Gardner K, et al.  Recruitment of p300/CBP in p53-dependent signal pathways. Cell. 1997;89:1175–1184
  20. Hallahan DE, Dunphy E, Virudachalam S, et al.  C-jun and Egr-1 participate in DNA synthesis and cell survival in response to ionizing radiation exposure. J Biol Chem. 1995;270:30303–30309
  21. Jung M, Zhang Y, Lee S, et al.  Correction of radiation sensitivity in ataxia telangiectasia cells by a truncated I kappa B-alpha. Science. 1995;268:1619–1621
  22. Yang CR, Wilson-Van Patten C, Planchon SM, et al.  Coordinate modulation of Sp1, NF-kappa B, and p53 in confluent human malignant melanoma cells after ionizing radiation. FASEB J. 2000;14:379–390
  23. Jung M, Zhang Y, Dimtchev A, et al.  Impaired regulation of nuclear factor-kappaB results in apoptosis induced by gamma radiation. Radiat Res. 1998;149:596–601
  24. Bakker TR, Reed D, Renno T, et al.  Efficient adenoviral transfer of NF-kappaB inhibitor sensitizes melanoma to tumor necrosis factor-mediated apoptosis. Int J Cancer. 1999;80:320–323
  25. Jeggo PA, Carr AM, Lehmann AR. Splitting the ATM (Distinct repair and checkpoint defects in ataxia-telangiectasia). Trends Genet. 1998;14:312–316
  26. Lavin MF, Khanna KK. ATM (The protein encoded by the gene mutated in the radiosensitive syndrome ataxia-telangiectasia). Int J Radiat Biol. 1999;75:1201–1214
  27. Uhrhammer N, Fritz E, Boyden L, et al.  Human fibroblasts transfected with an ATM antisense vector respond abnormally to ionizing radiation. Int J Mol Med. 1999;4:43–47
  28. Zhang N, Chen P, Gatei M, et al.  An anti-sense construct of full-length ATM cDNA imposes a radiosensitive phenotype on normal cells. Oncogene. 1998;17:811–818
  29. Petrini JH, Bressan DA, Yao MS. The RAD52 epistasis group in mammalian double strand break repair. Semin Immunol. 1997;9:181–188
  30. Essers J, Hendriks RW, Swagemakers SM, et al.  Disruption of mouse RAD54 reduces ionizing radiation resistance and homologous recombination. Cell. 1997;89:195–204
  31. Park MS. Expression of human RAD52 confers resistance to ionizing radiation in mammalian cells. J Biol Chem. 1995;270:15467–15470
  32. Vispe S, Cazaux C, Lesca C, et al.  Overexpression of Rad51 protein stimulates homologous recombination and increases resistance of mammalian cells to ionizing radiation. Nucleic Acids Res. 1998;26:2859–2864
  33. Ohnishi T, Taki T, Hiraga S, et al.  In vitro and in vivo potentiation of radiosensitivity of malignant gliomas by antisense inhibition of the RAD51 gene. Biochem Biophys Res Commun. 1998;245:319–324
  34. Cheong N, Wang X, Wang Y, et al.  Loss of S-phase-dependent radioresistance in irs-1 cells exposed to x-rays. Mutat Res. 1994;314:77–85

PII: S0360-3016(00)01497-8

International Journal of Radiation Oncology * Biology * Physics
Volume 49, Issue 2 , Pages 367-371 , 1 February 2001

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