International Journal of Radiation Oncology * Biology * Physics
Volume 73, Issue 3 , Pages 869-877 , 1 March 2009

Differential Effects of X-Rays and High-Energy 56Fe Ions on Human Mesenchymal Stem Cells

  • Kyle Kurpinski, Ph.D.

      Affiliations

    • Department of Bioengineering, University of California–Berkeley, Berkeley, CA
  • ,
  • Deok-Jin Jang, Ph.D.

      Affiliations

    • Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA
  • ,
  • Sanchita Bhattacharya, M.S.

      Affiliations

    • Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA
  • ,
  • Bjorn Rydberg, Ph.D.

      Affiliations

    • Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA
  • ,
  • Julia Chu, B.S.

      Affiliations

    • Department of Bioengineering, University of California–Berkeley, Berkeley, CA
  • ,
  • Joanna So, B.S.

      Affiliations

    • Department of Bioengineering, University of California–Berkeley, Berkeley, CA
  • ,
  • Andy Wyrobek, Ph.D.

      Affiliations

    • Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA
  • ,
  • Song Li, Ph.D.

      Affiliations

    • Department of Bioengineering, University of California–Berkeley, Berkeley, CA
  • ,
  • Daojing Wang, Ph.D.

      Affiliations

    • Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA
    • Corresponding Author InformationReprint requests to: Daojing Wang, Ph.D., Life Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, MS 977-225A, Berkeley, CA 94720. Tel: (510) 486-6592; Fax: (510) 495-2535

Received 12 May 2008 ,Revised 29 August 2008 ,Accepted 3 September 2008.

References 

  1. Grove JE, Bruscia E, Krause DS. Plasticity of bone marrow-derived stem cells. Stem Cells. 2004;22:487–500
  2. Ducy P. Cbfa1: A molecular switch in osteoblast biology. Dev Dyn. 2000;219:461–471
  3. Beausejour CM, Campisi J. Ageing: Balancing regeneration and cancer. Nature. 2006;443:404–405
  4. Rubio D, Garcia-Castro J, Martin MC, et al. Spontaneous human adult stem cell transformation. Cancer Res. 2005;65:3035–3039
  5. Serakinci N, Guldberg P, Burns JS, et al. Adult human mesenchymal stem cell as a target for neoplastic transformation. Oncogene. 2004;23:5095–5098
  6. Hall EJ, Hei TK. Genomic instability and bystander effects induced by high-LET radiation. Oncogene. 2003;22:7034–7042
  7. Morgan WF. Is there a common mechanism underlying genomic instability, bystander effects and other nontargeted effects of exposure to ionizing radiation?. Oncogene. 2003;22:7094–7099
  8. Schimmerling W, Cucinotta FA, Wilson JW. Radiation risk and human space exploration. Adv Space Res. 2003;31:27–34
  9. Nelson GA. Fundamental space radiobiology. Gravit Space Biol Bull. 2003;16:29–36
  10. Birrell GW, Brown JA, Wu HI, et al. Transcriptional response of Saccharomyces cerevisiae to DNA-damaging agents does not identify the genes that protect against these agents. Proc Natl Acad Sci U S A. 2002;99:8778–8783
  11. Yin E, Nelson DO, Coleman MA, et al. Gene expression changes in mouse brain after exposure to low-dose ionizing radiation. Int J Radiat Biol. 2003;79:759–775
  12. Ding LH, Shingyoji M, Chen F, et al. Gene expression profiles of normal human fibroblasts after exposure to ionizing radiation: A comparative study of low and high doses. Radiat Res. 2005;164:17–26
  13. Sokolov MV, Smirnova NA, Camerini-Otero RD, et al. Microarray analysis of differentially expressed genes after exposure of normal human fibroblasts to ionizing radiation from an external source and from DNA-incorporated iodine-125 radionuclide. Gene. 2006;382:47–56
  14. Wang D, Park JS, Chu JS, et al. Proteomic profiling of bone marrow mesenchymal stem cells upon transforming growth factor β stimulation. J Biol Chem. 2004;279:43725–43734
  15. Kurpinski K, Chu J, Hashi C, et al. Anisotropic mechanosensing by mesenchymal stem cells. Proc Natl Acad Sci U S A. 2006;103:16095–16100
  16. Zeitlin C, Heilbronn L, Miller J. Detailed characterization of the 1087 MeV/nucleon ion-56 beam used for radiobiology at the alternating gradient synchrotron. Radiat Res. 1998;149:560–569
  17. Groesser T, Chun E, Rydberg B. Relative biological effectiveness of high-energy iron ions for micronucleus formation at low doses. Radiat Res. 2007;168:675–682
  18. Baumann P, West SC. Role of the human RAD51 protein in homologous recombination and double-stranded-break repair. Trends Biochem Sci. 1998;23:247–251
  19. Maiorano D, Lutzmann M, Mechali M. MCM proteins and DNA replication. Curr Opin Cell Biol. 2006;18:130–136
  20. Pruitt SC, Bailey KJ, Freeland A. Reduced Mcm2 expression results in severe stem/progenitor cell deficiency and cancer. Stem Cells. 2007;25:3121–3132
  21. Xiong Y, Hannon GJ, Zhang H, et al. p21 is a universal inhibitor of cyclin kinases. Nature. 1993;366:701–704
  22. Rydberg B, Cooper B, Cooper PK, et al. Dose-dependent misrejoining of radiation-induced DNA double-strand breaks in human fibroblasts: Experimental and theoretical study for high- and low-LET radiation. Radiat Res. 2005;163:526–534
  23. Lucke-Huhle C, Blakely EA, Chang PY, et al. Drastic G2 arrest in mammalian cells after irradiation with heavy-ion beams. Radiat Res. 1979;79:97–112
  24. Rieger K, Marinets O, Fietz T, et al. Mesenchymal stem cells remain of host origin even a long time after allogeneic peripheral blood stem cell or bone marrow transplantation. Exp Hematol. 2005;33:605–611
  25. Dickhut A, Schwerdtfeger R, Kuklick L, et al. Mesenchymal stem cells obtained after bone marrow transplantation or peripheral blood stem cell transplantation originate from host tissue. Ann Hematol. 2005;84:722–727
  26. Chen MF, Lin CT, Chen WC, et al. The sensitivity of human mesenchymal stem cells to ionizing radiation. Int J Radiat Oncol Biol Phys. 2006;66:244–253
  27. Fei P, El-Deiry WS. P53 and radiation responses. Oncogene. 2003;22:5774–5783
  28. Waldman T, Kinzler KW, Vogelstein B. p21 is necessary for the p53-mediated G1 arrest in human cancer cells. Cancer Res. 1995;55:5187–5190
  29. Bunz F, Dutriaux A, Lengauer C, et al. Requirement for p53 and p21 to sustain G2 arrest after DNA damage. Science. 1998;282:1497–1501
  30. Matsuoka S, Ballif BA, Smogorzewska A, et al. ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. Science. 2007;316:1160–1166
  31. Franceschi RT, Xiao G. Regulation of the osteoblast-specific transcription factor, Runx2: Responsiveness to multiple signal transduction pathways. J Cell Biochem. 2003;88:446–454
  32. Lengner CJ, Steinman HA, Gagnon J, et al. Osteoblast differentiation and skeletal development are regulated by Mdm2-p53 signaling. J Cell Biol. 2006;172:909–921
  33. Koshurnikova NA, Gilbert ES, Sokolnikov M, et al. Bone cancers in Mayak workers. Radiat Res. 2000;154:237–245
  34. Rosemann M, Lintrop M, Favor J, et al. Bone tumorigenesis induced by alpha-particle radiation: Mapping of genetic loci influencing predisposition in mice. Radiat Res. 2002;157:426–434
  35. Oghiso Y, Yamada Y. The specific induction of osteosarcomas in different mouse strains after injections of 239Pu citrate. J Radiat Res (Tokyo). 2003;44:125–132
  36. Virtanen A, Pukkala E, Auvinen A. Incidence of bone and soft tissue sarcoma after radiotherapy: A cohort study of 295,712 Finnish cancer patients. Int J Cancer. 2006;118:1017–1021
  37. Hamilton SA, Pecaut MJ, Gridley DS, et al. A murine model for bone loss from therapeutic and space-relevant sources of radiation. J Appl Physiol. 2006;101:789–793
  38. Willey JS, Grilly LG, Howard SH, et al. Bone architectural and structural properties after 56Fe26+ radiation-induced changes in body mass. Radiat Res. 2008;170:201–207
  39. Cucinotta FA, Durante M. Cancer risk from exposure to galactic cosmic rays: Implications for space exploration by human beings. Lancet Oncol. 2006;7:431–435

 Conflict of interest: none.

 Supplementary material for this article can be found at www.redjournal.org.

PII: S0360-3016(08)03527-X

doi: 10.1016/j.ijrobp.2008.10.002

International Journal of Radiation Oncology * Biology * Physics
Volume 73, Issue 3 , Pages 869-877 , 1 March 2009