International Journal of Radiation Oncology * Biology * Physics
Volume 73, Issue 3 , Pages 861-868 , 1 March 2009

Neoplastic Transformation Induced by Carbon Ions

  • Daniela Bettega, Ph.D.

      Affiliations

    • Dipartimento di Fisica, Università di Milano and Istituto Nazionale di Fisica Nucleare (INFN), Milano, Italy
    • Corresponding Author InformationReprint requests to: Daniela Bettega, Ph.D., Dipartimento di Fisica, Via Celoria 16-20133 Milano, Italy. Tel: ++390250317210; Fax: ++390250317630
  • ,
  • Paola Calzolari, Ph.D.

      Affiliations

    • Dipartimento di Fisica, Università di Milano and Istituto Nazionale di Fisica Nucleare (INFN), Milano, Italy
  • ,
  • Petra Hessel, M.T.A.

      Affiliations

    • Biophysik, Gesellschaft für Schwer Ionenforschung (GSI), Darmstadt, Germany
  • ,
  • Claudio G. Stucchi, Ph.D.

      Affiliations

    • Istituto Nazionale dei Tumori, Milano, Italy
  • ,
  • Wilma K. Weyrather, Ph.D.

      Affiliations

    • Biophysik, Gesellschaft für Schwer Ionenforschung (GSI), Darmstadt, Germany

Received 16 November 2007 ,Revised 4 July 2008 ,Accepted 23 July 2008.

References 

  1. Schulz-Ertner D, Nikoghosyan A, Thilmann C, et al. Results of carbon ion radiotherapy in 152 patients. Int J Radiat Oncol Biol Phys. 2004;58:631–640
  2. Schulz-Ertner D, Karger CP, Feuerhake A, et al. Effectiveness of carbon ion radiotherapy in the treatment of skull-base chordomas. Int J Radiat Oncol Biol Phys. 2007;68:449–457
  3. Hawkins MM. Second primary tumors following radiotherapy for childhood cancer. Int J Radiat Oncol Biol Phys. 1990;19:1297–1301
  4. Heyn R, Haeberlen V, Newton WA, et al. Second malignant neoplasm in children treated for rhabdomyosarcoma. Intergroup Rhabdomyosarcoma Study Committee. Clin Oncol. 1993;11:262–270
  5. Sznajder L, Abrahams C, Parry D, et al. Multiple schwannomas and meningiomas associated with irradiation in childhood. Arch Intern Med. 1996;156:1873–1878
  6. Reznikoff CA, Brankow DW, Heidelberger C. Establishment and characterization of a clone line of C3H mouse embryo cells sensitive to postconfluence inhibition of division. Cancer Res. 1973;33:3231–3238
  7. Hei TK, Komatsu K, Hall EJ, et al. Oncogenic transformation by charged particles of defined LET. Carcinogenesis. 1988;9:747–750
  8. Miller RC, Hall EJ. Oncogenic transformation of C3H 10T1/2 cells by acute and protracted exposures to monoenergetic neutrons. Radiat Res. 1991;128(Suppl 1):S60–S64
  9. Balcer-Kubiczek EK, Harrison GH, Hei TK. Neutron dose-rate experiments at the AFRRI Nuclear Reactor. Radiat Res. 1991;128(Suppl 1):S65–S70
  10. Bettega D, Calzolari P, Pollara P, et al. In vitro cell transformations induced by 31 MeV protons. Radiat Res. 1985;104:178–181
  11. Bettega D, Calzolari P, Noris Chiorda G, et al. Transformation of C3H 10T1/2 cells with 4.3 MeV α particles at low doses: Effects of single and fractionated doses. Radiat Res. 1992;131:66–71
  12. Miller RC, Marino A, Brenner DJ, et al. The biological effectiveness of radon-progeny alpha particles. II. Oncogenic transformation as a function of linear energy transfer alpha particles. Radiat Res. 1995;142:54–60
  13. Yang TC-H, Craise LM, Mei M-T, et al. Neoplastic cell transformation by heavy charged particles. Radiat Res. 1985;104(Suppl 8):S177–S187
  14. Miller RC, Marino SA, Napoli J, et al. Oncogenic transformation in C3H10T1/2 cells by low-energy neutrons. Int J Radiat Biol. 2000;76:327–333
  15. International Commission on Radiation Units and Meaurements. The Quality Factor in Radiation Protection. Report 40. International Commission on Radiation Units and Meaurements. Washington, DC; 1986.
  16. International Commission on Radiation Protection. 1990 Recommendations of the International Commission on Radiation Protection. Publication 60. Annals of the ICRP 21. London: Pergamon; 1991;
  17. Stanbridge EJ, Der CJ, Doersen CJ, et al. Human cell hybrids analysis of transformation and tumorigenicity. Science. 1982;215:252–259
  18. Redpath JL, Sun C, Colman M, et al. Neoplastic transformation of human hybrid cells by radiation: A quantitative assay. Radiat Res. 1987;110:468–472
  19. Mendonca MS, Antoniono RJ, Latham KM, et al. Characterization of intestinal alkaline phosphatase expression and the tumorigenic potential of γ-irradiated HeLa x fibroblast cell hybrids. Cancer Res. 1991;51:4455–4462
  20. Mendonca MS, Antoniono RJ, Sun C, et al. A simplified and rapid staining method for the HeLa x skin fibroblast human hybrid cell neoplastic transformation assay. Radiat Res. 1992;131:345–350
  21. Bettega D, Calzolari P, Piazzolla A, et al. Alpha-particle-induced neoplastic transformation in synchronized hybrid cells of HeLa and human skin fibroblasts. Int J Radiat Biol. 1997;72:523–529
  22. Weyrather WK, Ritter S, Scholz M, et al. RBE for carbon track-segment irradiation in cell lines of differing repair capacity. Int J Radiat Biol. 1999;75:1357–1364
  23. Sun C, Redpath JL, Colman M, et al. Further studies on the radiation-induced expression of a tumor-specific antigen in human cell hybrids. Radiat Res. 1988;114:84–93
  24. Han A, Elkind MM. Transformation of mouse C3H10T1/2 cells by single and fractionated doses of X-rays and fission-spectrum neutrons. Cancer Res. 1979;39:123–130
  25. Kraft G, Daues HW, Fischer B, et al. Irradiation chamber and sample changer for biological samples. Nucl Instrum Methods. 1980;168:175–179
  26. Kraft-Weyrather W, Kraft G, Ritter S, et al. The preparation of biological targets for heavy-ion experiments up to 20 MeV/u. Nucl Instrum Methods. 1989;282:22–27
  27. Haberer Th, Becher W, Schardt D, et al. Magnetic scanning system for heavy ion therapy. Nucl Instrum Methods. 1993;330:296–305
  28. Schwab T. Transport von Schwerionen durch Materie innerhalb ionenoptischer Systeme. Ph.D. thesis, Universitát Giessen. GSI Report 1991;91–10.
  29. Bettega D, Calzolari P, Doglia B, et al. Cell thickness measurements by confocal fluorescence microscopy on C3H10T1/2 and V79 cells. Int J Radiat Biol. 1998;74:397–403
  30. Jaekel O, Hartmann GH, Karger CP, et al. A calibration procedure for beam monitors in a scanned beam of heavy charged particles. Med Phys. 2004;31:1009–1013
  31. Jäkel O, Hartmann G, Karger C, et al. Quality assurance for a treatment planning system in scanned ion beam therapy. Med Phys. 2000;27:1588–1600
  32. Colman M, Bhatt S, Candelaria M, et al. A comparison of the radiation sensitivities of non-tumorigenic and tumorigenic human hybrid cell lines. Int J Radiat Biol. 1988;53:609–616
  33. Frankenberg D, Kelnholer K, Bar K, et al. Enhanced neoplastic transformation by mammography X rays relative to 200kVp X rays: Indication for a strong dependence on photon energy of the RBEM for various end points. Radiat Res. 2002;157:99–105
  34. Frankenberg-Schwager M, Spieren S, Pralle E, et al. Neoplastic transformation of a human hybrid cell line by alpha particles in relation to mammography X rays. Radiat Prot Dosimetry. 2006;122:180–184
  35. Elmore E, Lao X-Y, Ko M, et al. Neoplastic transformation in vitro induced by low doses of 232 MeV protons. Int J Radiat Biol. 2005;81:291–297
  36. Redpath JL, Antoniono RJ, Sun C, et al. Late mitosis/early G1 phase and mid–G1 phase are not hypersensitive cell cycle phases for neoplastic transformation of hela x skin fibroblast human hybrid cells induced by fission-spectrum neutrons. Radiat Res. 1995;141:37–43
  37. Han RC, Suzuki H, Suzuki F, et al. Neoplastic transformation of hamster embryo cells by heavy ions. Adv Space Res. 1998;22:1725–1732
  38. Borek C, Hall EJ, Rossi HH. Malignant transformation in cultured hamster embryo cells produced by X-rays, 430-keV monoenergetic neutrons, and heavy ions. Cancer Research. 1978;38:2997–3005
  39. Suzuki M, Watanabe M, Suzuki K, et al. Neoplastic cell transformation by heavy ions. Radiat Res. 1989;120:468–476
  40. Martin S, Miller RC, Geard CR, et al. The biological effectiveness of radon-progeny alpha particles. IV. Morphological transformation of Syrian hamster embryo cells at low doses. Radiat Res. 1995;142:70–77
  41. Hall EJ. Failla Memorial Lecture; From beans to genes—back to the future. Radiat Res. 1992;129:235–249
  42. Trott DA, Cuthbert AP, Overell RW, et al. Mechanisms involved in the immortalization of mammalian cells by ionizing radiation and chemical carcinogens. Carcinogenesis. 1995;16:193–204
  43. Mendonca MS, Howard K, Fasching CL, et al. Loss of suppressor loci on chromosomes 11 and 14 may be required for radiation-induced neoplastic transformation on HeLa x fibroblast human cell hybrids. Radiat Res. 1998;149:246–255
  44. Alpen EL, Powers-Risius P, Curtis SB, et al. Fluence-based relative biological effectiveness for charged particles carcinogenesis in mouse Harderian gland. Adv Space Res. 1994;14:573–581
  45. Imaoka T, Nishimura M, Kakinuma S, et al. High relative biologic effectiveness of carbon ion radiation on induction of rat mammary carcinoma and its lack of H-Ras and TP53 mutations. Int J Radiat Oncol Biol Phys. 2007;69:194–203
  46. Hall EJ, Wuu C-S. Radiation-induced second cancers: The impact of 3D-CRT and IMRT. Int J Radiat Oncol Biol Phys. 2003;56:83–88
  47. Hall EJ. Intensity-modulated radiation therapy, protons, and the risk of second cancers. Int J Radiat Oncol Biol Phys. 2006;65:1–7

 This research project was supported in part by the European Commission under the 6th Framework Programme through the Integrated Infrastructure Initiative on EUROpean Nuclear Structure (EURONS), Contract No. Rii3-CT-2004-506065, and by the Italian Space Agency (Contract MO-MA 1 B1341-X5 COUNT).

 Conflict of interest: none.

PII: S0360-3016(08)03598-0

doi: 10.1016/j.ijrobp.2008.07.067

International Journal of Radiation Oncology * Biology * Physics
Volume 73, Issue 3 , Pages 861-868 , 1 March 2009